2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Sibstans estanda yo itilize nan koub kalibrasyon distribisyon mas molekilè relatif la: ensilin, mikopeptid, glisin-glisin-tirozin-arjinin, glisin-glisin-glisin
3 Enstriman ak ekipman
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
An jeneral, pwopòsyon asid amine nan pwodwi Sustar yo pi wo pase sa ki nan pwodwi Zinpro yo.
Pati 8 Efè itilizasyon
Efè diferan sous mineral tras sou pèfòmans pwodiksyon ak kalite ze poul ponnèz nan fen peryòd ponn lan.
Pwosesis Pwodiksyon
Teknoloji kelasyon siblé
Teknoloji emulsifikasyon tayisman
Teknoloji espre ak siye anba presyon
Teknoloji refrijerasyon ak dezumidifikasyon
Teknoloji avanse pou kontwòl anviwònman an
Apendis A: Metòd pou Detèminasyon distribisyon mas molekilè relatif peptid yo
Adopsyon estanda: GB/T 22492-2008
1 Prensip Tès:
Li te detèmine pa kwomatografi filtrasyon jèl pèfòmans segondè. Sa vle di, lè l sèvi avèk yon filler pore kòm faz estasyonè, ki baze sou diferans nan gwosè mas molekilè relatif konpozan echantiyon yo pou separasyon, detekte nan lyezon peptidik longèdonn absòpsyon iltravyolèt 220nm, lè l sèvi avèk lojisyèl pwosesis done dedye pou detèminasyon distribisyon mas molekilè relatif pa kwomatografi filtrasyon jèl (sa vle di, lojisyèl GPC a), kwomatogram yo ak done yo te trete, kalkile pou jwenn gwosè mas molekilè relatif peptid soya a ak seri distribisyon an.
2. Reyaktif
Dlo eksperimantal la ta dwe satisfè spesifikasyon dlo segondè nan GB/T6682, itilizasyon reyaktif yo, eksepte pou dispozisyon espesyal, yo dwe pi bon kalite pou analiz.
2.1 Reyaktif yo enkli asetonitril (kromatografikman pi), asid trifluoroacetic (kromatografikman pi),
2.2 Sibstans estanda yo itilize nan koub kalibrasyon distribisyon mas molekilè relatif la: ensilin, mikopeptid, glisin-glisin-tirozin-arjinin, glisin-glisin-glisin
3 Enstriman ak ekipman
3.1 Kromatograf Likid Pèfòmans Segondè (HPLC): yon estasyon travay kromatografik oswa yon entegratè ak yon detektè UV ak yon lojisyèl tretman done GPC.
3.2 Inite filtraj ak degazaj vakyòm faz mobil.
3.3 Balans elektwonik: valè gradye 0.000 1g.
4 etap operasyon yo
4.1 Kondisyon kromatografik ak eksperyans adaptasyon sistèm (kondisyon referans)
- 4.1.1 Kolòn kromatografik: TSKgelG2000swxl300 mm × 7.8 mm (dyamèt enteryè) oubyen lòt kolòn jèl menm kalite a ki gen pèfòmans sanblab epi ki apwopriye pou detèminasyon pwoteyin ak peptid.
- 4.1.2 Faz mobil: Asetonitril + dlo + asid trifluoroasetik = 20 + 80 + 0.1.
- 4.1.3 Longèdonn deteksyon: 220 nm.
- 4.1.4 Debi: 0.5 mL/min.
- 4.1.5 Tan deteksyon: 30 min.
- 4.1.6 Volim enjeksyon echantiyon an: 20μL.
- 4.1.7 Tanperati kolòn: tanperati chanm.
- 4.1.8 Pou sistèm kromatografik la ka satisfè egzijans deteksyon yo, yo te stipile ke anba kondisyon kromatografik ki anwo yo, efikasite kolòn kromatografik jèl la, sa vle di kantite plak teorik (N), pa dwe mwens pase 10000 kalkile sou baz pik estanda tripeptid la (Glycine-Glycine-Glycine).
- 4.2 Pwodiksyon koub estanda mas molekilè relatif yo
- Solisyon estanda peptid ki gen diferan mas molekilè relatif ki anwo yo, avèk yon konsantrasyon mas 1 mg/mL, te prepare pa matche faz mobil, melanje nan yon sèten pwopòsyon, epi filtre nan yon manbràn faz òganik ak yon gwosè pò 0.2 μm ~ 0.5 μm epi enjekte nan echantiyon an, epi answit jwenn kromatogram estanda yo. Koub kalibrasyon mas molekilè relatif yo ak ekwasyon yo te jwenn lè yo trase logaritm mas molekilè relatif la an fonksyon de tan retansyon an oswa pa regresyon lineyè.
4.3 Tretman echantiyon an
Peze avèk presizyon 10mg echantiyon an nan yon fiole volumetrik 10mL, ajoute yon ti kras faz mobil, souke echantiyon an avèk ultrasons pandan 10 minit, pou echantiyon an fonn nèt epi melanje, dilye ak faz mobil lan selon balans lan, epi filtre atravè yon manbràn faz òganik ki gen yon gwosè pò 0.2μm ~ 0.5μm, epi analize filtrat la dapre kondisyon kromatografik ki nan A.4.1.
- 5. Kalkil distribisyon mas molekilè relatif la
- Apre analiz solisyon echantiyon an prepare nan 4.3 anba kondisyon kwomatografik 4.1 yo, nou ka jwenn mas molekilè relatif echantiyon an ak ranje distribisyon li lè nou ranplase done kwomatografik echantiyon an nan koub kalibrasyon 4.2 la avèk lojisyèl pwosesis done GPC. Nou ka kalkile distribisyon mas molekilè relatif diferan peptid yo avèk metòd normalizasyon zòn pik la, dapre fòmil sa a: X=A/A total×100
- Nan fòmil la: X - Fraksyon mas yon peptid ki gen yon mas molekilè relatif nan total peptid ki nan echantiyon an, %;
- A - Sifas pik yon peptid ki gen yon mas molekilè relatif;
- Total A - sòm sifas pik chak peptid ki gen mas molekilè relatif, kalkile avèk yon sèl plas desimal.
- 6 Repetabilite
- Diferans absoli ant de detèminasyon endepandan ki jwenn anba kondisyon repetabilite pa dwe depase 15% nan mwayèn aritmetik de detèminasyon yo.
- Apendis B: Metòd pou Detèminasyon Asid Amine Lib yo
- Adopsyon estanda: Q/320205 KAVN05-2016
- 1.2 Reyaktif ak materyèl
- Asid asetik glasyal: pi bon kalite analitik
- Asid pèklorik: 0.0500 mol/L
- Endikatè: 0.1% endikatè kristal vyolèt (asid asetik glasyal)
- 2. Detèminasyon asid amine lib yo
Echantiyon yo te seche nan 80°C pandan 1 èdtan.
Mete echantiyon an nan yon veso sèk pou l refwadi natirèlman nan tanperati chanm oubyen pou l refwadi jiskaske li ka itilize.Peze apeprè 0.1 g echantiyon an (ak yon presizyon 0.001 g) nan yon flakon konik sèk 250 mL.Ale byen vit nan pwochen etap la pou evite echantiyon an absòbe imidite anbyen an.Ajoute 25 mL asid asetik glasyal epi melanje byen pou pa plis pase 5 minit.Ajoute 2 gout endikatè kristal vyolètTitre ak 0.0500 mol / L (±0.001) solisyon titrasyon estanda asid pèklorik jiskaske solisyon an chanje soti nan koulè mov pou rive nan pwen final la.
Anrejistre volim solisyon estanda ki konsome a.
- Fè tès vid la an menm tan.
- 3. Kalkil ak rezilta yo
- Kontni asid amine lib X nan reyaktif la eksprime kòm yon fraksyon mas (%) epi li kalkile dapre fòmil sa a: X = C × (V1-V0) × 0.1445/M × 100%, nan fòmil la:
- C - Konsantrasyon solisyon asid pèklorik estanda an mòl pa lit (mol/L)
- V1 - Volim ki itilize pou titrasyon echantiyon yo ak solisyon asid pèklorik estanda, an mililit (mL).
- Vo - Volim ki itilize pou titrasyon vid ak solisyon asid pèklorik estanda, an mililit (mL);
M - Mas echantiyon an, an gram (g).
| 0.1445: Mas mwayèn asid amine ekivalan a 1.00 mL solisyon asid pèklorik estanda [c (HClO4) = 1.000 mol / L]. | 4.2.3 Solisyon titrasyon estanda silfat seryòm: konsantrasyon c [Ce (SO4) 2] = 0.1 mol/L, prepare dapre GB/T601. | |
| Adopsyon estanda yo: Q/70920556 71-2024 | 1. Prensip detèminasyon (Fe kòm egzanp) | Konplèks fè asid amine yo gen yon solubilite ki ba anpil nan etanòl anid epi iyon metal lib yo soluble nan etanòl anid, diferans nan solubilite ant de yo nan etanòl anid te itilize pou detèmine to kelasyon konplèks fè asid amine yo. |
| Nan fòmil la: V1 - volim solisyon estanda silfat seryòm ki konsome pou titrasyon solisyon tès la, mL; | Etanòl anhid; rès la se menm jan ak kloz 4.5.2 nan GB/T 27983-2011. | 3. Etap analiz yo |
| Fè de esè an paralèl. Peze 0.1g echantiyon an ki te seche a 103±2℃ pandan 1 èdtan, avèk yon presizyon 0.0001g, ajoute 100mL etanòl anid pou l fonn, filtre, lave rezidi filtre a avèk 100mL etanòl anid omwen twa fwa, answit transfere rezidi a nan yon flakon konik 250mL, ajoute 10mL solisyon asid silfirik dapre kloz 4.5.3 nan GB/T27983-2011, epi answit fè etap sa yo dapre kloz 4.5.3 "Chofe pou fonn epi kite l refwadi" nan GB/T27983-2011. Fè tès vid la an menm tan. | 4. Detèminasyon kontni total fè a | 4.1 Prensip detèminasyon an se menm jan ak kloz 4.4.1 nan GB/T 21996-2008 la. |
4.2. Reyaktif ak Solisyon
| 4.2.1 Asid melanje: Ajoute 150mL asid silfirik ak 150mL asid fosfòrik nan 700mL dlo epi melanje byen. | 4.2.2 Solisyon endikatè sodyòm difenilamin silfonat: 5g/L, prepare dapre GB/T603. | 4.2.3 Solisyon titrasyon estanda silfat seryòm: konsantrasyon c [Ce (SO4) 2] = 0.1 mol/L, prepare dapre GB/T601. | |
| 4.3 Etap analiz yo | Fè de esè an paralèl. Peze 0.1g echantiyon an, avèk yon presizyon 020001g, mete l nan yon flakon konik 250mL, ajoute 10mL asid melanje, apre disolisyon an, ajoute 30ml dlo ak 4 gout solisyon endikatè sodyòm dianilin silfonat, epi swiv etap sa yo dapre kloz 4.4.2 nan GB/T21996-2008. Fè tès vid la an menm tan. | 4.4 Reprezantasyon rezilta yo | Yo kalkile kontni total fè X1 nan konplèks fè asid amine yo an tèm de fraksyon mas fè a, valè a eksprime an %, dapre fòmil (1) an: |
| X1=(V-V0)×C×M×10-3×100 | V0 - solisyon estanda silfat seryòm ki konsome pou titrasyon solisyon vid la, mL; | V0 - solisyon estanda silfat seryòm ki konsome pou titrasyon solisyon vid la, mL; | C - Konsantrasyon aktyèl solisyon estanda silfat seryòm, mol/L5. Kalkil kontni fè nan kelat yoYo kalkile kontni fè X2 nan kelat la an fonksyon fraksyon mas fè a, valè a eksprime an %, dapre fòmil sa a: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Nan fòmil la: V1 - volim solisyon estanda silfat seryòm ki konsome pou titrasyon solisyon tès la, mL; | V2 - solisyon estanda silfat seryòm ki konsome pou titrasyon solisyon vid la, mL;nom1-Mas echantiyon an, g. Pran mwayèn aritmetik rezilta detèminasyon paralèl yo kòm rezilta detèminasyon yo, epi diferans absoli rezilta detèminasyon paralèl yo pa plis pase 0.3%. | 0.05585 - mas fè fereuz eksprime an gram ekivalan a 1.00 mL solisyon estanda silfat seryòm C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Mas echantiyon an, g. Pran mwayèn aritmetik rezilta detèminasyon paralèl yo kòm rezilta detèminasyon yo, epi diferans absoli rezilta detèminasyon paralèl yo pa plis pase 0.3%. | 6. Kalkil pousantaj kelasyon anTo kelasyon X3, valè a eksprime an %, X3 = X2/X1 × 100Apendis C: Metòd pou Detèminasyon To Kelasyon Zinpro a |
Adopsyon estanda: Q/320205 KAVNO7-2016
1. Reyaktif ak materyèl
a) Asid asetik glasyal: pi bon kalite pou analiz; b) Asid pèklorik: 0.0500mol/L; c) Endikatè: 0.1% endikatè kristal vyolèt (asid asetik glasyal)
2. Detèminasyon asid amine lib yo
2.1 Echantiyon yo te seche a 80°C pandan 1 èdtan.
2.2 Mete echantiyon an nan yon veso sèk pou l refwadi natirèlman nan tanperati chanm oubyen pou l refwadi jiskaske li ka itilize.
2.3 Peze apeprè 0.1 g echantiyon (ak yon presizyon 0.001 g) nan yon flakon konik sèk 250 mL.
2.4 Ale byen vit nan pwochen etap la pou evite echantiyon an absòbe imidite anbyen an.
2.5 Ajoute 25mL asid asetik glasyal epi melanje byen pou pa plis pase 5min.
2.6 Ajoute 2 gout endikatè kristal vyolèt.
2.7 Titre ak yon solisyon titrasyon estanda 0.0500mol/L (±0.001) asid pèklorik jiskaske solisyon an chanje soti nan koulè mov rive nan vèt pandan 15s san li pa chanje koulè kòm pwen final.
2.8 Anrejistre volim solisyon estanda ki konsome a.
2.9 Fè tès vid la an menm tan.
- 3. Kalkil ak rezilta yo
- Katalan
- Physicochemical parameters
V1 - Volim ki itilize pou titrasyon echantiyon yo ak solisyon asid pèklorik estanda, an mililit (mL).
Vo - Volim ki itilize pou titrasyon vid ak solisyon asid pèklorik estanda, an mililit (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adrès: No.147 Qingpu Road, Shouan Town, Pujiang County, chengdu City, Sichuan Province, Lachin
Telefòn: 86-18880477902
Pwodwi yo
Mineral tras inòganik
- Mineral tras òganik yo
- Swahili
- Sèvis pèsonalize
- Lyen rapid yo
Pwofil Konpayi an
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Klike pou fè demann | © Dwa otè - 2010-2025 : Tout dwa rezève. | Plan sit la PI GWO RECHÈCH Telefòn |
| Telefòn | 86-18880477902 | Javanè | Imèl |
| 8618880477902 | Chinwa | Franse | |
| Bird | Chinwa | Franse | Alman Panyòl |
| Aquatic animals | Japonè | Koreyen | Arab Grèk |
| Tik | Italyen | ||
| Ruminant animal g/head day | January 0.75 | Endonezyen Afrikans Syèd |
Polonè
- Bask
- Katalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bilgaryen
- Sebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kwoasyen
Olandè
| Application object | Ourdou Vyetnamyen | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Ayisyen | Aoussa | Kinyarwanda Hmong Ongwa |
| Piglets and fattening pigs | Igbo | Javanè | Kannada Khmer Kurd |
| Kirghiz | Latin | ||
| Bird | 300～400 | 45～60 | Masedonyen Malay Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Nòvejyen
- Pachto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Sèb
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tajik
Tamil
Telugu
Thai
| Application object | Ourdou Vyetnamyen | Content in full-value feed (mg/kg) | Efficacy |
| Yidich | Yoruba | Zoulou | Kinyarwanda Oriya Turkmen |
| Ouïgour | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
